METHOD FOR THE PURIFICATION OF LEUCOCYTOSIS-PROMOTING FACTOR HEMAGGLUTININ (LPF-HA)

摘要

- 1 -AbstractThe invention provides a method for producinghighly pure LPF-HA (Leucocytosis-promoting FactorHemagglutinin) from the culture media of Bordetella pertussisin a high yield by an affinity chromatography using a denaturedceruloplasmin as a ligand. The LPF-HA obtained by thisinvention is highly pure and does not contain endotoxins ofB. pertussis and is useful for the preparation of variousreagents utilizing its physiological activities, as a medicinefor treating diabetes, and as a pertussis vaccine.