Process for production of extracellular keratinolytically active protease

摘要

A process for production of extracellular keratinolytically active protease is typical in that the growing medium containing keratinous or some other complex proteinous material as inductor of enzymatic synthesis, complex components of microbiological culture media, simple sources of carbon, and inorganic salts as a source of minerals, with its starting pH in a slightly acidic up to neutral range, is sterilized and cooled down to about 30 degrees Centigrade, then grafted with spores of the fungus Doratomyces Microsporus to obtain the culture concentration of 108 - 109 spores/L, grown in submersion aerobic conditions at the temperature of 25 up to 30 degrees Centigrade under shaking, fermented for 50 up to 60 hours, after which the slurry is filtered and the enzyme of the following characteristics is isolated from the filtrate: molecular weight 33 kDa, activity against keratinous skin in the pH range between 6 and 12, and activity against keratinous skin in the temperature range between 20 and 55 degrees Centigrade. The applied fungus Doratomyces Microsporus is preserved in the national collection of microbic cultures (Collection Nationale de Cultures de Microorganismes - CNCM, INSTITUT PASTEUR) in Paris under the designation MZKI B399 and registered on December the 23rd 1997 under reg. No. I-1963.