REGULATION OF GENE EXPRESSION IN EUKARYOTES

摘要

There is provided a method of regulating a eukaryotically active gene, comprising transforming a cell with a transformation cassetteexpressing a modulator gene poduct regulating the eukaryotically gene or its product and a further gene product regulating said modulatorgene or its product, the promoters of two of said gene, modulator gene and further genes being selected from inducible promoters anddevelopmental promoters for the same or complementary issues. The lethal gene expressing barnase, a ribonuelease of B. amiloliquefaciens,is placed undet the control of a tissue specific promoter, such as those derived from PrMADS1, 2 or 3 of Pinus radiata or EGM1, 2 or 3of Eucalytus grandis. The same tisssue specific promoter is used to express Laciq gene, a repressor for barnase (barsiar) being promotedby a modified 35s RNA CaMY promoter including the lac operon. The cassette is used to transform plant cells for regeneration into plantsexpressing the barnase in the target tissues with improved specificity and reduced promoter leakage.