Regulation of gene expression in eukaryotes.

摘要

Invention patent: b "regulation of gene expression in eukaryotes" d .It is provided a method of controlling a gene eucarioticamente active.Which comprises transforming a cell with a package of processing which expresses a gene product regulates the eucarioticamente modulator gene or its product and another gene product whichRegulates the said gene or its product modulator, the promoters of two of said gene.The modulator gene and other gene being selected induzu00edveis promoters and promoters of the development for the same or additional tissues.The lethal gene expressing barnase, a ribonuclease from Mu B.Amyloliquefaciens MV, is placed under the control of a tissue specific promoter, such as those derived from prmads1, 2 or 3 Mu Pinus radiata MV or egm1,2 or 3 Mu Eucalyptus grandis MV.The same tissue specific promoter is used to express the gene laclq barnase (barstar, a repressive to being promoted by) a promoter of CaMV 35S RNA modified including opu00e9ron of lac.The package is used to transform plant cells for the regeneration of plants expressing the barnase in the tissue target with improved specificity and leakage of promoter is reduced.