Procedure for obtaining ribosome inactivating proteins, called Beetin Beetin 27 and 29, leaves of Beta vulgaris (sugarbeet) infected with the virus of yellowness.

摘要

Procedure for obtaining ribosome-inactivating proteins, called Beetin Beetin 27 and 29, leaves Beta vulgaris (sugar beet) infected with the virus yellowness. a process for obtaining two ribosome inactivating proteins called beetins 27 and 29 of the Beta vulgaris plant described from an extract obtained from the intercellular fluid leaves Beta vulgaris infected with virus and not from a plant extract total. The general procedure for obtaining the beetins of the present invention includes the steps below: a) Preparation of extract intercellular fluid leaves the plant. b) Dialysis and development of ion exchange chromatography of the protein obtained in the previous step with a salt gradient. c) Gel permeation chromatography of protein peaks from the previous stage. Such proteins have use as inhibitors of protein and as biosíntesisde inactivating genomic virus ribonucleic acid and have utility in cancer therapy and AIDS and viral plant diseases.