A method of high loading of vesicles with biopolymer substances.

摘要

A METHOD FOR HIGHER FREIGHT vesicles SUBSTANCES biopolymer FUNCTIONAL IN BIOCHEMICAL SYSTEMS OR HUMAN PHYSIOLOGICAL RESULTING FROM ANY SOURCE, these substances preferably being enzymes, proenzymes, cofactors, PARTICLE submicron AS virions, growth factors, cytokines, ROBOSOMAS, ANTIGEN SURFACE HEPATITIS B, oligo- and polynucleotides, antibodies and / or antigens, SUCH vesicles FORMED BY SUBSTANCES amphipathic comprising the steps of A) a mixture of lipids suitable for forming vesicles lipid solvent ORGANICOS water immiscible alternatively MAY BE USED LIPIDOS DRIED OR lIPID MIXTURE IN ANY FORM (POWDER, GRANULAR, etc.) directly; B) Desolventising IN THE PRESENCE OF A SOLID SUPPORT; C) bringing the product of step b) to a solution of the substances to be encapsulated in A physiologically compatible solution; D) adding an organic solvent having properties of SOLUBILIZACIONO provision and E) DESICCATE the fraction obtained in step d) under conditions WITHHOLDING OF THE ROLE OF SUBSTANCES encapsulated.