SELECTABLE MARKER FOR DEVELOPMENT OF VECTORS AND TRANSFORMATION SYSTEMS IN PLANTS

摘要

The present invention discloses functional andselectable micro-Ti plasmids. The hygromycinphosphotransferase (aphIV) gene from Escherichia coliwas inserted between the 5' promoter and associatedamino terminal region-encoding sequence of an octopinesynthetase gene and the 3' terminator signal sequenceof a nopaline synthetase gene. These constructs wereassembled between T-DNA border fragments in abroad-host-range vector and used to createantibiotic-resistant plant cells.