Selectable marker for development of vectors and transformation systems in plants

摘要

The present invention discloses functional and selectable micro-Ti plasmids. The hygromycin phosphotransferase (aphIV) gene from Escherichia coli was inserted between the 5' promoter and associated amino terminal region-encoding sequence of an octopine synthetase gene and the 3' terminator signal sequence of a nopaline synthetase gene. These constructs were assembled between T-DNA border fragments in a broad- host- range vector and used to create antibiotic-resistant plant cells.