PROCESS FOR PREPARING RECOMBINANT PROTEINS USING HIGHLY EFFICIENT EXPRESSION VECTOR FROM $i(SACCHAROMYCES CEREVISIAE)

摘要

This invention concerns a method of producing human granulocyte colony-stimulating factor (hGCSF) and human growth hormone (hGH) from yeast by using recombinant DNA technology. More specifically, this invention relates to a method of producing hGCSF or hGH by using yeast expression vector which contains: hybrid promoter comprising promoters of two different yeast-derived genes, yeast killer toxin leader peptide, and amino terminus of IL-1β. In addition, this invention relates to a method of producing hGCSF by using expression vector which contains promoter and secretion signal of HSP 150.