Method for synthesizing stable, single-stranded cDNA in eukaryotes by transfection with a vector containing a bacterial retron and the vector used therefor

摘要

A method for producing in vivo stable single-stranded DNAs in eucaryotic cells. The DNAs are multicopy single-stranded DNA (msDNA) structures constituted by an RNA and a DNA portion. The group of genes (retrons) producing said coupled RNA and DNA portions of the msDNAs and the gene encoding reverse transcriptase (RT). The transformed eucaryotes harboring these retrons. The new msDNAs which are encoded by the new retrons. Utilities are disclosed.