GENERATION OF VIRAL TRANSFECTANTS USING RECOMBINANT DNA-DERIVED NUCLEOCAPSID PROTEINS

摘要

This invention provides a method of producing viral transfectants (viruses containing a heterologous nucleic acid) that eliminates the need for purified RNP complexes or for purified viral RNA polymerases. The method generally involves: (i) combining a ribonucleic acid (nucleic acid transcript) with an isolated nucleoprotein (NP) of the virus to form a synthetic ribonucleoprotein complex (RNP); (ii) transfecting a host cell with the synthetic ribonucleoprotein complex; and (iii) holding (culturing) the host cell under conditions permitting replication of the virus.