Expression vector for the conditional regulation of expression of the large subunit of polymerase II

摘要

An expression vector (A) for eukaryotic cells is new and comprises at least: (1) the gene (I) for the large subunit (X) of RNA-polymerase II (Pol II), or its mutants, that are resistant to alpha -amantine (aA), or its derivatives; (2) a sequence (II) for conditional regulation of expression of (I); (3) sequence (IV) allowing selection of the vector in prokaryotic and/or eukaryotic cells; (4) a sequence (V) allowing replication of the vector in such cells and (5) a polyadenylation site (PAA). Independent claims are also included for the following: (1) prokaryotic or eukaryotic cells containing (A); and (2) a method for conditional, stable expression of (X) by introducing (A) into a eukaryotic cell, selection in absence of aA and with inhibition of (I) expression, induction of (I) expression and addition of aA to block expression of the endogenous, nonresistant Pol II.