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An expression vector for the conditioned enable position regulation of the expression of the large subunit of the rna - polymerase ii

机译:用于条件调节的表达载体能够位置调节rna-聚合酶ii大亚基的表达

摘要

an expression vector for eukaryontenzellen erfindungsgemu00e4u00df is provided, at least, the following elements: $a (a) the gene for the large subunit of rna polymerase ii or mutated forms of the rna polymerase ii and the mutated forms of against - ama nitin or a derivative thereof are resistant; $a (b) nucleotide sequences.the regulation of expression of the large subunit remaining conditional statements of the amanitin resistant rna polymerase ii gene or the mutated forms; $a (c) nucleotide sequences to the selection of the vector in prokaryotes and / or eukaryotic cells; $a (d) on the replication of nucleic acid sequences of the vector in prokaryotes cells and / or eukaryotic cells; $a (e) a polyadenylierungsstelle (p(aa).
机译:提供了真核生物的表达载体,至少包含以下元件:$ a(a)rna聚合酶ii的大亚基的基因或rna聚合酶ii的突变形式以及针对-的突变形式ama nitin或其衍生物具有抗性; $ a(b)核苷酸序列。对大的亚基表达的调节,其仍然是抗金黄色素抗核糖核酸聚合酶ii基因或突变形式的条件陈述; (a)在原核细胞和/或真核细胞中选择载体的核苷酸序列; $ a(d)关于载体的核酸序列在原核细胞和/或真核细胞中的复制; $ a(e)聚腺苷酸双酯(p(aa)。

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