Expression vector for the conditional regulation of expression of the large subunit of polymerase II

摘要

According to the invention, an expression vector for eukaryotic cells is provided which includes at least the following elements: (A) The gene for the large subunit of RNA polymerase II or mutated forms thereof, wherein the RNA polymerase II or mutated forms thereof against α-amanitin or a derivative thereof are resistant; (B) nucleotide sequences which enable a conditional regulation of the expression of the large subunit of the α-amanitin-resistant RNA polymerase II gene, or the mutant forms; (C) nucleotide sequences for selection of the vector in prokaryotic and / or eukaryotic cells; (D) nucleotide sequences for replication of the vector in prokaryotic cells and / or eukaryotic cells; (E) a polyadenylation site (PAA).