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Expression Vector of Human Growth Hormone and Method of Manufacturing Human Growth Hormone Using the Same
Expression Vector of Human Growth Hormone and Method of Manufacturing Human Growth Hormone Using the Same
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机译:人生长激素的表达载体及使用该表达载体制备人生长激素的方法
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摘要
The present invention relates to an expression vector of human growth hormone and a method for producing human growth hormone using the same. More specifically, the present invention provides a L-arabinose comprising a fragment of glutathione S-transferase, a DNA fragment encoding a urokinase cleavage site and a human growth hormone gene. ) Inducible expression vectors, recombinant microorganisms transformed therefrom, and electric microorganisms are cultured in a medium to which L-arabinose is added to induce expression, thereby producing a large amount of human growth hormone. The fusion protein prepared from the expression vector of the present invention is produced in high yield through precise L-arabinose induction regulation, and contains an amino acid sequence specifically cleaved by urokinase between the fusion partner and human growth hormone. By treatment with, it is possible to effectively separate the recombinant human growth hormone that retains the activity of the native human growth hormone as it is from the fusion protein.
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