Fluorescent silkworm and manufacturing method using recombinant baculovirus with green fluorescent protein gene inserted

摘要

The present invention is to produce a recombinant virus (Bacterial Accession No. KCTC0418BP) inserted into the green fluorescent protein gene under the control of the polyhedral protein gene promoter of insect baculovirus (AcNPV), silkworm body cavity injection The present invention relates to a fluorescent silkworm and a method for producing a recombinant silkworm, in which a green fluorescent protein gene is expressed by small-scale proliferation of a recombinant virus and regulation of a polyhedral protein gene promoter. The degree of expression of fluorescent silkworms differed according to the silkworm varieties. Especially, Daesunggum was effective in the expression rate and metastasis, and green fluorescence was emitted all over the body during ultraviolet irradiation. In addition, the green fluorescent protein gene of fluorescent silkworms was transferred to the genome by the mechanism of insertion into the genome of host insects. Therefore, the fluorescent silkworm development technology of the present invention can be utilized for the creation of transgenic insects with improved new function and genetic engineering.