freezing method of dog sperm and artificial insemination method utilizing the sperm and compound for freezing the sperm thereof

摘要

The invention purpose is to centrifuge the semen collection process, and the collected semen to collect semen in sutkae leaving only drift down to the lower end, a mixture of semen separation process and, the same amount of semen with the reagent to filter the fluid in the upper portion the primary refrigeration was then frozen to hold the mixing process, and mixed with the reagent fixed to chilled in the refrigerator car 2 which again mix only the same quantity of reagents and the next tube was frozen and mixed into the liquid nitrogen in a refrigerator of about 5 ℃ courses consist of two semen freezing method consists of.; Further Thus inject thawing process, and to test the vitality of the thawed sperm vitality of procedure and sperm into the reagent dissolving comprising a refrigerated semen with sodium chloride and the serum in the vagina of the female dogs and formed on a straight-line injection process of massaging the clitoris by this method to achieve artificial insemination, cryopreservation, which is of sodium citrate per 0.25~4.5g semen 100㎖, 0.45~4.0g per priority, 0.01~0.3g penicillin, streptomycin 50,000 who not only to 50 IU, Catalyst eluent 0.45~0.25g, 0.045~0.25g yolk, glycerol 1.5~15%, and preserving the excellent blood of varieties by making possible the development of a cryopreservation agent composition consisting of serum, and every year two 1500-2000 ( You can stop the tremendous waste of foreign currency due to more imports of foreign varieties of the Ministry of Agriculture and Forestry statistics) to produce one of the excellent pedigree reverse the possible export features Have.