Compositions for freezing dog sperm freezing methods of the dog sperm utilizing the compositions and artificial insemination method empolying the freezed dog sperm

摘要

The present invention relates to a composition for freezing semen of dogs and a freezing method using the same, and also to an artificial insemination method using semen frozen by the above-mentioned freezing method. The present invention per 100ml of distilled water, sodium citrate 0.25 to 4.5g, dextrose 0.45 to 4.0g, penicillin 0.01 to 0.3g, streptomycin 50,000 to 500,000 IU, catalase 0.25 to 0.45g, egg yolk 0.045 to 0.25g, glycerol 1.5 A composition for freezing canine semen containing from 15 ml to 15 ml and dog serum from 0.02 to 0.1 μg is provided. In addition, the present invention (a) semen collecting step of collecting the semen of the male by the massage method or the electric shock method; (b) centrifuging the collected semen to filter the fluid of the upper layer and semen separation step taking only the lower end; (c) first refrigeration of the semen at the bottom and the composition as described above in a dose ratio of 1: 1; (d) further adding the composition as described above to the mixture produced by step (c), mixing and secondary refrigeration so that the volume ratio of the semen and composition is 1: 2; and (e) the (d) It provides a method of freezing the semen of the dog comprising the step of adding liquid nitrogen to the mixture produced by the step) and freezing.