detection of reverse transcriptase with dna hybridization

摘要

A method for detecting reverse transcriptase (RT), utilizing RT-catalyzed generation of cDNA complementary to an RNA template and detecting cDNA in a DNA hybridization reaction, is provided. Also provided is a method for detecting and/or quantitating drug resistance of reverse transcriptase in a sample and a method for evaluating ability of a compound to inhibit reverse transcriptase activity.