Methods for identification or purification of cells containing an enzymatic intracellular marker

摘要

Production of a stem cell enriched cell composition using essentially non-toxic methodology comprising contacting a cell mixture with a cell- permeable, fluorescent non-polar compound that reacts upon contact with intracellular enzyme aldehyde dehydrogenase or phosphokinase found substantially exclusively in stem cells so as to form a biocompatible fluorescent product that is polar and non-permeable to the membrane of the stem cells, and isolating the cells containing the marker by fluorescent cell sorting.