Identifying sequence differences in nucleic acid populations

摘要

Methods of identifying sequence differences between or among nucleic acid populations are described. Nucleic acid strands from different populations are hybridized with one another so that mismatch- containing duplexes are formed. Mismatch-containing duplexes are cleaved in a mismatch-dependent fashion and cleavage products are isolated and used to identify the genetic sequences that differ in the nucleic acid populations.