Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities

摘要

The invention provides for a methods and compositions for identifying proteins or compounds that directly or indirectly modulate a genomic polynucleotide and methods for identifying active genomic polynucleotides. Generally, the method comprises inserting a BL (beta- lactamase) expression construct into an eukaryotic genome, usually non- yeast, contained in at least one living cell, contacting the cell with a predetermined concentration of a modulator, and detecting BL activity in the cell.