METHODS AND COMPOSITIONS FOR SENSITIVE AND RAPID, FUNCTIONAL IDENTIFICATION OF GENOMIC POLYNUCLEOTIDES AND USE FOR CELLULAR ASSAYS IN DRUG DISCOVERY

摘要

The invention provides for methods and compositions for identifying proteins or chemicals that directly or indirectly modulate a genomic polynucleotide and methods for identifying active genomic polynucleotides. Generally, the method comprises inserting a BL (beta-lactamase) expression construct into an eukaryotic genome, usually non-yeast, contained in at least one living cell, contacting the cell with a predetermined concentration of a modulator, and detecting BL activity in the cell.