Measurement techniques that can be used to determine with high sensitivity mass of phosphatidylinositol 3,4,5-triphosphate

摘要

In a method of test (PtdIns (3,4,5) P ₃₎ and phosphatidylinositol 3,4,5-triphosphate (57) [summary] sample, and phosphatidylinositol that exist in (a) sample - It was converted to inositol 1,3,4,5-tetraphosphate 3,4,5 triphosphate and inositol-1, the amount of (b) inositol 1,3,4,5-tetraphosphate is measured by binding to a polypeptide that selectively binds to the four 3,4,5-phosphate. Preferably, phosphatidylinositol 3,4,5-triphosphate is converted to inositol 1,3,4,5-tetraphosphate by alkaline hydrolysis.