AN ASSAY WHICH MEASURES PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE MASS AT HIGH SENSITIVITY

摘要

A method of assaying phosphatidylinositol 3,4,5 trisphosphate (Ptd Ins(3,4,5)P3) in a sample, the method comprising the steps of: (a) converting phosphatidylinositol 3,4,5 trisphospate present in the sample to inositol 1,3,4,5 tetrakisphosphate (Ins(1,3,4,5)P4); and (b) measuring the amount of inositol 1,3,4,5 tetrakisphosphate. Conveniently, the amount of inositol 1,3,4,5 tetrakisphosphate is measured by binding to a polypeptide which binds inositol 1,3,4,5 tetrakiphosphate selectively. Preferably, the phosphatidylinositol 3,4,5 trisphosphate is converted to inositol 1,3,4,5 tetrakisphosphate by alkaline hydrolysis.