Due to be transformed with the cDNA of trehalose-6-phosphate synthase / phosphatase Seraginera Repidofira-derived method to expand trehalose content in the organism

摘要

(57) [summary] The present invention describes a measurement cloning and nucleotide sequence of the complete molecule of complementary DNA encoding a bifunctional enzyme trehalose-6-phosphate synthetase / phosphatase of plant. Complementary DNA molecule encodes a bifunctional enzyme that synthesizes trehalose. Enzymatic DNA can be subcloned into a suitable vector for the speech in a host cell. The production of trehalose in comparison with non-transformed cells, transformed cells to increase the resistance to the load induced heat, cold, and drying the salt concentration thereby. Can be used to improve the osmotic tolerance and heat resistance of plant to be grown in semi-arid areas and drying, the present invention contributes to reduction of the use of water watering purposes. In addition, trehalose was produced in transgenic plants to long-term storage under dehydrating conditions without having to extend the shelf life of agricultural products, After After dehydration, compromising the flavor characteristic aroma, and taste it. Finally, over-expression of trehalose bacteria, yeast, in transgenic plant or animal cells, constitute the economic source of this product to be used as a preservative in the preservation of processed foods or biological production of variety.