The culture solution for the neurocyte, the production manner and culture manner null of the neurocyte which uses

摘要

PROBLEM TO BE SOLVED: To obtain the subject culture liquid capable of stably performing the culture of central nerve cells by blending albumin to a supernatant of a culture collected by culturing primary astroglia cells in a nutrition medium added with insulin and transferrin. ;SOLUTION: This culture liquid for nerve cells capable of stably performing a culture of central nerve cells, excellent in extending property of neuraxon, capable of rapidly forming synapses in a low density culture, and obtaining the cells forming a neural network in a high density culture, is obtained by adding insulin and transferrin to one or more kinds of nutrition media such as Eagle basal medium(MEM), Dulbecco modified Eagle medium(DMEM) and Ham F-12 medium, culturing primary astroglia cells, then collecting the supernatant of the medium, blending albumin to the supernatant of the medium and further adding progesterone, insulin, transferrin, selenious acid (salt), a superoxide dismutase and a catalase thereto.;COPYRIGHT: (C)1997,JPO