Making use of the western horseradish peroxidase the method the sign of doing the organism molecule

摘要

(57) Abstract This invention uses the western horseradish peroxidase (HRP), peptide, it regards the enzyme sign method of organism molecule such as hormone, antibody and other hapten. The method of proposing, without controlling the heterogeneous connection product it is not to form, meta it passes the enzyme and oxidizes with the iodic acid sodium, with the style which next, is controlled, , it builds a bridge the -jiamino alkane (to be desirable, 1,13 - diamino - 4,7,10 - toriokisatoridekan) and between the molecules making use of how many of the aldehyde basis which was formed by the said oxidation. Because of this, the cohesion body which consists of 6 - 10 HRP molecules forms, does not separate from reaction blend beforehand and also the te, through the remaining aldehyde basis, can connect the protein which the sign it should do to the said cohesion body. Advantage of this kind of HRP building a bridge is to be able to achieve higher-level substitution. Because the said protein does not hide on back of the HRP molecule, it can show the immunology quality what without being restricted causing error furthermore making use of the technology of the spectrophotometer dying it can measure. Sensitivity and peculiarity of the HRP tracer which is obtained are high, completely equal to the radioactive tracer very. This way the HRP sign method of being proposed shows considerable advantage at the time of developing the enzyme immunology detection system of the high performance.