FRAGMENTATION AND PREPARATION OF SUBUNIT FROM 11S GLOBULIN OF SOYBEAN, AND ITS PRODUCT

摘要

PROBLEM TO BE SOLVED: To provide a method for readily and efficiently fractionating and preparing an acidic subunit and a basic subunit, safe in food sanitation without using a reducing agent on 11S globulin. ;SOLUTION: An acidic subunit fraction and a basic subunit fraction are fractionated by bringing 11S globulin which is a major component of the soybean protein into contact with steam at a temp. of ≥150°C (under about 4 kgf/cm2 gage pressure of the steam). The fractionation is carried out under one of conditions that no reducing agent is used at all at the time of the contact with the steam, a centrifugal separation or a filtration is carried out after the contact with the steam, the temperature is kept at a temp. of ≥120°C for ≥1 min after the contact with the steam, and the contact with the steam is performed at ≥10% concentration of the protein, and the like. As the result, the cost of the reducing agent becomes unnecessary, and the method enables the cost for the removing operation, the treating expense, etc., of the remaining sulfurous acid by the conventional technique to be reduced compared to the conventional technique. The method can contribute to mass production, etc.;COPYRIGHT: (C)2000,JPO