Transcriptome-Based Identification of a Seed Olive Legumin (11S Globulin). Characterization of Subunits, 3D Modelling and Molecular Assessment of Allergenicity

摘要

Seed storage proteins (SSPs) are fundamental molecules for seed germination as an important source of carbon and nitrogen. Among the main four protein families that integrate SSPs, legumins (11S globulins) are widely distributed in dicots, and represent the major contribution to the pool of seed proteins in olive. In the present study, we have used an olive seed transcriptome generated de novo by 454/Roche Titanium+ sequencing to identify a broad panel of US protein sequences. Among these identified legumin sequences, five were selected using their presence within the output results from the BLASTP alongside the whole NCBI database, and their clustering with previously-characterized US sequences in the phylogenetic analysis as the criteria. The selected sequences were identified as corresponding to the isoform 2 of the 11S protein precursor, and one of the sequences was used for further analysis. Individual acidic and basic subunits within this sequence were recognised, 3D-modeled and assessed as regard to their potential molecular interaction by docking methods. Furthermore, T-cell epitopes were forecast by using predictive software in order to evaluate the putative implications of the olive 11S proteins in food allergy. The potential use of this protein highly present in the olive seed as a food source is discussed.