PROTEIN HAVING ANTIGENICITY OF CANINE DISTEMPER VIRUS NUCLEOCAPSID PROTEIN AND REAGENT FOR ASSAYING ANTI-CANINE DISTEMPER VIRUS NUCLEOCAPSID PROTEIN ANTIBODY

摘要

PROBLEM TO BE SOLVED: To obtain a new protein by affording itself with the antigenicity of a specific nucleocapsid protein so as to enable anti-CDV-NP antibody to be assayed quickly and correctly without the need of any special equipment and therefore be useful in e.g. diagnosing CDV infection. ;SOLUTION: This new protein is such one as to have an average molecular weight of about 58 kD and have the antigenicity of nucleocapsid protein as one of the constitutive proteins of Canine Distemper Virus belonging to the genus morbillivirus, Paramyxoviridae. The protein can be taken from insect cells obtained by infecting insect-derived cells with recombinant baculovirus integrated with a DNA having a sequence complementary to the genetic domain encoding the protein and has an amino acid sequence of the formula. Furthermore, it is preferable that the other objective reagent is prepared by including, as active ingredient, an antigen formed by immobilizing the protein on a support or membrane.;COPYRIGHT: (C)1999,JPO