SIMULTANEOUS DETECTION OF CITRUS TATTER LEAF VIRUS AND CITRUS VIROID BY RT-PCR

摘要

PROBLEM TO BE SOLVED: To obtain a nucleic acid primer set capable of simultaneously detecting citrus tatter leaf virus, citrus exocortis viroid, etc., by combining a plurality of nucleic acid primer pairs having specific base sequences, respectively. ;SOLUTION: This nucleic acid primer set for simultaneously detecting three or more of citrus tatter leaf virus, citrus exocortis viroid, citrus viroids I, II, III and IV comprises at least three nucleic acid primer pairs selected from a nucleic acid primer pair comprising a nucleic acid primer containing a base sequence of formula I and a nucleic acid primer containing a base sequence of formula II, a nucleic acid primer pair comprising a nucleic acid primer containing a base sequence of formula III and a nucleic acid primer containing a base sequence of formula IV, a nucleic acid primer pair comprising a nucleic acid primer containing a base sequence of formula V and a nucleic acid primer containing a base sequence of formula VI, a nucleic acid primer pair comprising a nucleic acid primer containing a base sequence of formula VII and a nucleic acid primer containing a base sequence of formula VIII, etc.;COPYRIGHT: (C)2000,JPO