Direct procedure specific detection of potato spindle tuber viroid viroid and viroid citrus Exocortis immobilized by RT-PCR and real-time detection kit for targets.

摘要

a preparation process, preservation and analysis of samples of plant material for diagnosis and detection by molecular amplification based RT-real time PCR detailed. The procedure has been applied to detection of potato spindle tuber viroid pospiviroides Citrus exocortis viroid and. Samples are immobilized on nylon supports paper or by printing of fabrics or its sections or absorbed on paper or nylon when it comes to liquid samples. Immobilized samples can be stored at room temperature. The protocol analysis begins by extraction targets of the support with distilled water octoxynol or glycine buffer followed by analysis by RT-PCR in real time. the nucleotide sequences of primers and TaqMan probes molecular amplification in the detection kit is based are described.