MEASUREMENT OF GLYCOSAMINOGLYCAN OR GLYCOSAMINOGLYCAN BONDING MOLECULE AND MEASUREMENT KIT THEREOF

摘要

PROBLEM TO BE SOLVED: To stably measure a glycosaminoglycan bonding molecule and the like in a specimen by an ease operation by using a carrier fixedly carrying lipid bonding glycosaminoglycan on its surface. ;SOLUTION: Using a plate, in which lipid bonding glycosaminoglycan (L-GAG) is bonded to a carrier such as an immunoassay plate by using a lipid residue as an anchor molecule, glycosaminoglycan (GAG) or a GAG bonding molecule in a specimen is measured. As GAGs constituting L-GAG, hyaluronic acid, chondroitin, chondroitin sulfate, and a GAG fragment prepared by decomposing these GAGs by a GAG decomposing enzyme are available. As a lipid, a lipid, which has a functional group bonding GAG directly or via a spacer and can be fixed to the carrier, is used, and a primary amino group containing lipid or a phospholipid is suitable. Preferably, L-GAG is fixed to the carrier through a hydrophobic bond.;COPYRIGHT: (C)2000,JPO