Process for the production of recombinant proteìna gp120 and proteìna gp120 recobinante of human immunodeficiency vìrus

摘要

b invention patent for the "process for the production of recombinant proteu00ecna gp 120 and proteu00ecna gp 120 recombinant human immunodeficiency vu00ecrus" d .The present invention relates to protein gp 120 and the production process of protein gp 120 recombinant human immunodeficiency virus produced by genetic engineering techniques.To be used in the diagnosis of immunological techniques of diagnosis as well as ELISA, Western blot, etc., and a possible use as a vaccine in the prevention of infection.The DNA containing the gene coding for the envelope protein gp 120 was obtained using the following steps: DNA amplification, DNA fragmentation (1), (2) the purification of DNA, digestion of DNA, (3)(4) cloning, (5) binding of DNA to the vector, (6) the transformation of competent bacteria, selection of positive clones (7), (8) the production of the protein, (9) induction of the bacteria, (10) lysis of the bacterium.(11) the purification of the protein. (12)