Method for Early Detection of HPV-Associated Carcinomas or Severe Degree of HPV Caused Dysplasia

摘要

PCT No. PCT/DE96/00306 Sec. 371 Date Jan. 28, 1998 Sec. 102(e) Date Jan. 28, 1998 PCT Filed Feb. 23, 1996 PCT Pub. No. WO96/26293 PCT Pub. Date Aug. 29, 1996This invention relates to a method of detection of an mRNA having HPV and cellular sequences in a body sample, comprising the following steps: (a) obtaining a body sample; (b) isolating mRNA from the body sample of (a); (c) translating the mRNA of (b) into cDNA using a primer common for reverse transcription; (d) amplifying the cDNA of (c) by a PCR reaction with a 5' HPV primer and a 3' primer having sequences of the primer of (c); (e) cleaving the amplified cDNA of (d) with an endonuclease cleaving on the 5' side of the HPV polyadenylation sequence; (f) amplifying the non-cleaved cDNA of (e) with the primers of (d) or with "nested" primers; and (g) detecting the amplified cDNA of (f). Furthermore, this invention concerns the use of such a method of early detection of HPV-associated carcinomas and extreme dysplasias caused by HPV, respectively.