Method of early detection of HPV-associated carcinomas and extreme dysplasias caused by HPV

摘要

This invention relates to a method of detection of an mRNA having HPV and cellular sequences in a body sample, comprising the following steps:PP(a) obtaining a body sample;P P(b) isolating mRNA from the body sample of (a);PP (c) translating the mRNA of (b) into cDNA using a primer common for reverse transcription;PP(d) amplifying the cDNA of (c) by a PCR reaction with a 5' HPV primer and a 3' primer having sequences of the primer of (c);PP(e) cleaving the amplified cDNA of (d) with an endonuclease cleaving on the 5' side of the HPV polyadenylation sequence;PP(f) amplifying the non-cleaved cDNA of (e) with the primers of (d) or with "nested" primers; andPP(g) detecting the amplified cDNA of (f).PPFurthermore, this invention concerns the use of such a method of early detection of HPV- associated carcinomas and extreme dysplasias caused by HPV, respectively.