PROCESS FOR PRODUCING EPIDERMIS-SPECIFIC GROWTH FACTOR, THE DNS CODING THIS, THE ANTIBODIES PRODUCED AGAINST THEM, AND PHARMACEUTICAL COMPOSITIONS CONTAINING THE GROWTH FACTOR OR THE ANTIBODY

摘要

The method of the invention relates to substantially pure human keratinocyte growth factor (KGF) protein or KGF-like protein production having an apparent molecular weight of about 16-30 kilodaltons by NaDodSO4 / PAGE, with a specific activity of 3.4 × 104 units / mg protein, with 1 unit activity. is the amount that, under standard test conditions, induces half of the possible maximum stimulation of DNA-- synthesis in BALB / MK keratinocyte cells, and the growth factor mitogen activity only stimulates the epithelial cells, fibroblast cells. The invention also relates to a method for generating DNA segments encoding the above proteins, recombinant molecules comprising aDNA segments, to their transfected host cells, and to the preparation of the above-described protein-reactive antibodies, as well as to a method for the preparation of a pharmaceutical composition comprising fentoproteins or antibodies. ŕ