A recombinant plasmid pSRQ800 containing DNA encoding a protein designated as AbiE which in Lactococcus lactis increases resistance to phages by aborting infection by phages and method and use

摘要

An isolated DNA encoding a protein designated as AbiE contained in plasmid pSRQ800 which in a Lactococcus lactis increase resistance to phages by aborting infection of the Lactococcus lactis by phages. This can be deposited as NRRL-B-21443, which is in a 4.5kb EcoR1 segment contained in the plasmid pSRQ800. The DNA has the SEQ ID NO:1 which is 4467 nucleotides in length. Also claimed is a recombinant plasmid comprising pSRQ800 encoding a protein designated as AbiE, the plasmid which is a shuttle vector pSA3 or pNZ123 containing the DNA, bacterium harboring a recombinant plasmid containing DNA contained in plasmid pSRQ800 encoding a protein designated as AbiE which in a Lactococcus lactis increases resistance to phage by aborting infection of the lactis phages.