A METHOD FOR PRODUCING RECOMBINANT FACTOR VIII, RECOMBINANT FACTOR VIII, CULTURAL MEDIUM

摘要

Recombinant Factor VIII can be produced in relatively large quantities on a continuous basis from mammalian cells in the absence of any animal-derived proteins such as albumin by culturing the cells in a protein free medium supplemented with polyol copolymer Pluronic F-68, in the presence of trace metals such as copper. In very preferred embodiments, the medium includes copper sulfate, ferrous sulfate/EDTA complex, and salts of trace metals such as manganese, molybdenum, silicon, lithium and chromium.