首页>
外国专利>
METHOD FOR THE PRODUCTION OF RECOMBINANT FACTOR VIII, A RECOMBINANT FACTOR VIII PRODUCED BY THE METHOD, AND A CELL CULTURE MEDIUM FOR USE IN THE METHOD
METHOD FOR THE PRODUCTION OF RECOMBINANT FACTOR VIII, A RECOMBINANT FACTOR VIII PRODUCED BY THE METHOD, AND A CELL CULTURE MEDIUM FOR USE IN THE METHOD
展开▼
机译:重组因子VIII的生产方法,通过该方法生产的重组因子VIII以及用于该方法的细胞培养介质
展开▼
页面导航
摘要
著录项
相似文献
摘要
Recombinant Factor VIII can be produced in relatively large quantities on a continuous basis from mammalian cells in the absence of any animal-derived proteins such as albumin by culturing the cells in a protein free medium supplemented with polyol copolymers, preferably in the presence of trace metals such as copper. In very preferred embodiments, the medium includes a polyglycol known as Pluronic F-68, copper sulfate, ferrous sulfate/EDTA complex, and salts of trace metals such as manganese, molybdenum, silicon, lithium and chromium. With an alternative medium which included trace copper ions alone (without polyol copolymers) we were also able to enhance the productivity of Factor VIII in recombinant cells such as BHK cells that are genetically engineered to express Factor VIII.
展开▼