首页>
外国专利>
detection and differentiation of mycobacterium tuberculosis complex bacteria by direct oligotypisierung of various repetetiven segments
detection and differentiation of mycobacterium tuberculosis complex bacteria by direct oligotypisierung of various repetetiven segments
展开▼
机译:重复片段的直接寡核苷酸检测和鉴别结核分枝杆菌复杂细菌
展开▼
页面导航
摘要
著录项
相似文献
摘要
PCT No. PCT/NL94/00110 Sec. 371 Date Jan. 22, 1997 Sec. 102(e) Date Jan. 22, 1997 PCT Filed May 16, 1994 PCT Pub. No. WO95/31569 PCT Pub. Date Nov. 23, 1995A method and primary pair and kit for the in vitro amplification of nucleic acid using amplification primers in amplification reactions. There is used a pair of primers comprising oligonucleotide sequences complementary to a part of a Direct Repeat sequence of a microorganism belonging to the M tuberculosis complex of microorganisms, whereby hybridization to a Direct Repeat occurs and subsequently elongation of the hybridized primer takes place. The primers are such that elongation in the amplification reaction occurs for one primer in a 5' direction and for the other primer in a 3' direction.
展开▼