Detection and differentiation of mycobacterium tuberculosis complex bacteria by direct oligo classification of different repeating segments

摘要

PCT No. PCT/NL94/00110 Sec. 371 Date Jan. 22, 1997 Sec. 102(e) Date Jan. 22, 1997 PCT Filed May 16, 1994 PCT Pub. No. WO95/31569 PCT Pub. Date Nov. 23, 1995A method and primary pair and kit for the in vitro amplification of nucleic acid using amplification primers in amplification reactions. There is used a pair of primers comprising oligonucleotide sequences complementary to a part of a Direct Repeat sequence of a microorganism belonging to the M tuberculosis complex of microorganisms, whereby hybridization to a Direct Repeat occurs and subsequently elongation of the hybridized primer takes place. The primers are such that elongation in the amplification reaction occurs for one primer in a 5' direction and for the other primer in a 3' direction.