Selection of cytochrome P-450 reductase inhibitors with respect to ergosterol biosynthesis.

摘要

A method for shielding FUNGICIDE AGENTS IDENTIFIED POTENTIAL biochemical or chemicals that inhibit the formation of ergosterol ESTEROL FUNGICIDA for inhibiting cytochrome P450 REDUCTASE ENZYMES OR REFERRED reductase or protein involved in their biosynthesis. Test samples are incubated IN TWO CULTURES yeast strains, and the extent of growth inhibition is compared between SI. CULTURE IS supersensitive to inhibitors Cytochrome P450 Reductase: yeast strain typically contains a gene Cytochrome P450 Reductase DELETED OR INTERRUPTED. OTHER SENSITIVE CROP IS LESS COMPOUNDS DISPLAYING ENHANCED TYPICALLY permeability as seen in the mutant ERGO. In preferred embodiments, a protein inhibitor ELECTRON TRANSFER TO ASSOCIATE Cytochrome P450 REDUCTASE AS ketoconazole, miconazole or econazole is used as a positive control. Preferred embodiments employ MEDIA Solidified, and applied test samples DISCS OR WELLS, ASSETS ARE IDENTIFIED BY THE PRODUCTION OF A DIFFERENTIAL ZONE or about 8 MM OR MORE ON THE LAWN yeast cultures.