首页>
外国专利>
The isolation and enrichment of neural stem cells from uncultured tissue based on cell-surface marker expression
The isolation and enrichment of neural stem cells from uncultured tissue based on cell-surface marker expression
展开▼
机译:基于细胞表面标志物表达的未培养组织中神经干细胞的分离和富集
展开▼
页面导航
摘要
著录项
相似文献
摘要
The invention provides methods for the prospective identification, isolation, and self-renewal of neural stem cells from the mammalian peripheral nervous system and compositions of neural stem cells derived from uncultured tissue. Using flow-cytometry, neural crest derived cells of embryonic peripheral nerve were fractionated based on cell surface markers. The isolated p75+ P0 cells from embryonic sciatic nerve were phenotypically and functionally indistinguishable from neural crest stem cells (NCSCs) previously isolated from neural tube explant cultures. Furthermore, freshly isolated p75+ P0 cells gave rise to both neurons and glia when transplanted in vivo. Cell cycle analysis and BrdU labeling indicated that p75+ P0NCSCs persist in the peripheral nerve by undergoing self-renewing divisions after neural crest migration has ceased.
展开▼