Increased levels of miR-124 in human dental pulp stem cells alter the expression of neural markers

摘要

cqvip:Auditory neuropathy is the particular form of deafness in humans which cannot be treated by replacement therapy.Human dental pulp stem cells(hDPSCs)are derived from an ectomesenchymal neural crest cell population.Therefore,they possess a promising capacity for neuronal differentiation and repair.miR-124,a key regulator of neuronal development in the inner ear,is expressed at high levels in auditory and vestibular neurons.Here,we evaluated the possible effect of miR-124 in alteration of neural protein markers expression.Using quantitative reverse transcription-PCR(qRT-PCR)analyses and immunofluorescence staining,we studied the expression patterns of neural progenitor markers(Nestin,NOTCH1,and SOX2)and neural markers(b-tubulin Ⅲ,GATA-3,and peripherin)upon transfection of hDPSCs with miR-124.The qRT-PCR results showed that Nestin was upregulated 6 h post-transfection.In contrast,Nestin expression exhibited a decreasing trend 24 h and 48 h post-transfection.Higher levels of b-tubulin Ⅲ,6 h and 16 h post transfection in RNA level as compared with control cells,were determined in transfected DPSCs.However,b-tubulin-Ⅲ expression decreased 48 h post-transfection.The immunoflourescence results indicated that transfection of hDPSCs with miR-124,only affected Nestin among the studied neural progenitor and neural marker expression in protein level.