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Dna cassette encoding a multimer of a biologically active peptide and a cleavable linker attached thereto and process for preparing the biologically active peptide
Dna cassette encoding a multimer of a biologically active peptide and a cleavable linker attached thereto and process for preparing the biologically active peptide
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机译:编码生物活性肽的多聚体和与其连接的可裂解的接头的dna盒以及制备该生物活性肽的方法
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摘要
A biologically active peptide is prepared by: a) preparing an expression vector comprising a DNA cassette containing two or more tandem repeating units of a nucleotide sequence encoding a biologically active peptide and a linker peptide attached thereto, the linker peptide being cleavable by a protease or a chemical agent; b) transforming a microorganism with the expression vector; c) culturing the transformed microorganism to produce a multimeric peptide expressed by the DNA cassette; d) recovering and digesting the multimeric peptide with the protease or the chemical agent to obtain the biologically active peptide or an analog thereof carrying one or more amino acid residues originating from the linker peptide.
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