FRAGMENTS OF INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN AND INSULIN-LIKE GROWTH FACTOR, AND USES THEREOF

摘要

The present invention provides the portions of IGFBP and IGF peptides which account for IGF-IGFBP binding. Thus, the present invention provides an isolated IGF binding domain of an IGFBP or modifications thereof, which binds IGF with at least about the same binding affinity as the full length IGFBP. The present invention also provides an IGF antagonist that reduces binding of IGF to an IGF receptor. Further provided is a fragment of IGF or modification thereof, wherein the fragment or modification thereof binds to a binding domain of IGFBP. The present invention further provides a protein comple, comprising the IGF binding domain of IGFBP of the invention and the IGF fragment of the invention. The present invention provides methods of treating a subject with cancer and of preventing cancer in a subject, of treating a subject with a diabetic complication exacerbated by IGF and of preventing diabetic complications exacerbated by IGF comprising administering to the subject the IGF antagonist of the present invention. Also provided is a method of treating a subject with an ischemic injury or preventing an ischemic injury in a subject, comprising administering to the subject the IGF fragment or IGFBP antagonist of the invention.