METHOD FOR TREATMENT OF AN AQUEOUS FLUX BY ELECTROPULSATION OF A FIELD PARALLEL TO THE FLOW, PULSATION CHAMBER AND USES THEREOF

摘要

The invention relates to a method for treating an aqueous flux which is colonized by cells by means of a pulsed electric field which is applied to the flux, characterized in that the applied field is substantially parallel to the flow of the flux . The invention also relates to the use thereof in the transfer of nucleic acids (RNA,DNA, oligonucleotides) in cells, the transfer of proteins in cells, the extraction of molecules and cytoplasmic macromolecules contained in the cells, cell fusion and the production of hybrids and/or the insertion of membrane proteins. The invention further relates to an electropulsation chamber, a method for cellular destruction and a method for membrane permeabilization.