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Time resolved optical array detectors and CCD cameras for frequency domain fluorometry amp;/or phosphorimetry
Time resolved optical array detectors and CCD cameras for frequency domain fluorometry amp;/or phosphorimetry
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机译:时间分辨光学阵列检测器和CCD摄像机,用于频域荧光法和/或磷光法
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摘要
An apparatus for cross-correlation frequency domain fluorometry-phosphorimetry comprises a source of electromatic radiation and means for amplitude modulating the radiation at the first frequency. The amplitude modulated radiation is directed at a sample, while an optical array detector measures the resulting luminescence of the sample. A signal is provided coherent with the amplitude modulated electromagnetic radiation signals, at a second frequency which is different from the first frequency. The apparatus has the capability for shutting off and turning on the coherent signal at the second frequency in a cycle having a third frequency which is different from the difference between the first and second frequencies. This produces a resultant signal at a frequency derived from the frequency difference and the third frequency. Typically the resultant signal is no more than about a quarter of the frequency difference. The resultant signal modulates the gain of the detecting means or multiplies its output, depending upon the nature of the detecting means. The amount of luminescence measured by the optical array detector is read when the signal is off in its cycle of the third frequency.
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